Washing E.coli cells/ osmotic pressure determination
ofb at wanadoo.fr
Tue Oct 12 13:35:01 EST 1999
We are currently washing E. coli cells with a water solution containing 9 g/
l of NaCl. However, it seems that we have some cell lysis.
I was wondering if it was an osmotic pressure problem? Do you know a simple
methode to measure an osmotic pressure? Do you know if I must modify the
concentration of NaCl that i'm using.
Thank you for your help.
oliver.favre-bulle at wanadoo.fr
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