cultivation of Leuconostoc mesenteroides

A. Seoane seoaneb at yahoo.com
Thu Oct 14 16:44:56 EST 1999


Hi, Ricki

As a clinical microbiologist, I do not find L.
mesenteroides too often, but when I do it grows quite
easily on sheep-blood agar or choc-agar incubated at
37ºC-24h in normal atmosphere. Maybe that it takes
more than 24h to grow at 28ºC.Take it easy.(Or maybe
it doesn´t like tomato at all....:)).


--- Frederik Boernke
<boernke at nospam.ipk-gatersleben.de> wrote:
> Hi,
> 
> I'd would like to cultivate L. mesenteroides in
> order to prepare
> DNA. I obtained some freeze-dried stuff from the
> DSMZ  which I 
> plated onto acidic tomato medium. However, I can not
> observe any
> growth after 24 h at 28 deg.. My textbook knowledge
> tells me that
> those bugs do actually ferment, but should be
> aerotolerant. 
> Any hints are appreciated.
> 
> Ricky
> 
>
*******************************************************************
> 
> Frederik Boernke
> Research Group of  Molecular Plant Physiology
> Institute for Plant Genetics and Crop Plant Research
> (IPK)
> Corrensstr. 3
> 06466 Gatersleben
> Tel.  039482 -5 321
> Fax. 039482 -5 515
> http://www.ipk-gatersleben.de
> 


=====

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