Got a Question for you regarding RNase A!

Anson Li ansonli at infoserve.net
Mon Oct 18 02:17:36 EST 1999


Hi Microbio people!

RNase is prepared by making the appropriate concentration in buffer,
heating it to 100 degrees for 15 minutes, and then allowed to cool slowly
to room temperature.  It is stored at -20 degrees celsius.

Is the heating process there to get rid of the oxygen?  But it doesn't
seem to make sense unless RNase was inhibited by oxygen???
And the storing of the protein in -20 degrees celsius is there because
RNase degrades slowly at room temperature???  Or is there
another reason?

Your input is appreciated.





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