Got a Question for you regarding RNase A!

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Oct 18 10:34:13 EST 1999


In article <kQzO3.51$Z64.1335372 at news1.van.metronet.ca>, Anson Li
<ansonli at infoserve.net> writes
>Is the heating process there to get rid of the oxygen?  But it doesn't
>seem to make sense unless RNase was inhibited by oxygen???
>And the storing of the protein in -20 degrees celsius is there because
>RNase degrades slowly at room temperature???  Or is there
>another reason?

To destroy DNase contaminants in the batch of pancreatic RNase A. Rnase
is highly stable but the DNase will be irreversibly denatured above 65C.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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