Got a Question for you regarding RNase A!

Dr. Duncan Clark Duncan at
Mon Oct 18 10:34:13 EST 1999

In article <kQzO3.51$Z64.1335372 at>, Anson Li
<ansonli at> writes
>Is the heating process there to get rid of the oxygen?  But it doesn't
>seem to make sense unless RNase was inhibited by oxygen???
>And the storing of the protein in -20 degrees celsius is there because
>RNase degrades slowly at room temperature???  Or is there
>another reason?

To destroy DNase contaminants in the batch of pancreatic RNase A. Rnase
is highly stable but the DNase will be irreversibly denatured above 65C.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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