Got a Question for you regarding RNase A!

A.J.Cann nna at le.ac.uk
Mon Oct 18 06:18:10 EST 1999


In article <kQzO3.51$Z64.1335372 at news1.van.metronet.ca>, Anson Li
<ansonli at infoserve.net> wrote:

> RNase is prepared by making the appropriate concentration in buffer,
> heating it to 100 degrees for 15 minutes, and then allowed to cool slowly
> to room temperature.  It is stored at -20 degrees celsius.
> 
> Is the heating process there to get rid of the oxygen?  But it doesn't
> seem to make sense unless RNase was inhibited by oxygen???
> And the storing of the protein in -20 degrees celsius is there because
> RNase degrades slowly at room temperature???  Or is there
> another reason?

The heating is done to denature any contaminating DNase activity, which
is heat-sensitive.

-- 
--
Dr Alan J. Cann  PhD,   Department of Microbiology & Immunology,
University of Leicester,  P.O. Box 138,  Medical Sciences Building,
University Road, Leicester LE1 9HN,  UK.



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