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Staining Staphylococcus

reddy kesav kreddy1 at GL.UMBC.EDU
Tue Oct 19 16:24:16 EST 1999


Hi
I am a engineering student study bacterial adhesion to collagen under
shear conditions in a perfusion chamber
I am trying to stain Staphylococcus aureus with Acridine Orange to count
the bacteria which adhere under flow. Unfortunately the dye leaks and also
photobleaches rapidly.
Could anyone suggest a different dye or a suitable protocol for Acridine
Orange?
Thanks Kesav 




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