How to block/destroy Fc domain of an antibody?

Tammy Ferguson tjfergus at hsc.vcu.edu
Wed Oct 27 11:29:06 EST 1999


Protein A will work for IgGs only and I'm not sure if it is subtype
specific (IgG1, IgG2a,b, etc.)

Goran Pavlakovic wrote:

> Hi there,
>
> does anybody have an idea how could I block/destroy Fc domain of an
> antibody (primary antibody) so that it would not be recognised by
> (otherwise) appropriate secondary antibody?
>
> Would incubation with Protein A do the trick?
>
> Thanx,
>
> Goran
>
> pavlakovic at gmx.net




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