How to block/destroy Fc domain of an antibody?
gtamura at u.washington.edu
Wed Oct 27 11:53:42 EST 1999
Protein A will definitely not do the trick. It binds only to a small
portion of the Fc domain, and won't block a significant portion of your
secondary antibody. What you need to do is make Fab fragments using
pepsin. Protocols are available readily in textbooks.
On Wed, 27 Oct 1999, Goran Pavlakovic wrote:
> Hi there,
> does anybody have an idea how could I block/destroy Fc domain of an
> antibody (primary antibody) so that it would not be recognised by
> (otherwise) appropriate secondary antibody?
> Would incubation with Protein A do the trick?
> pavlakovic at gmx.net
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