How to block/destroy Fc domain of an antibody?

Glen Tamura gtamura at u.washington.edu
Wed Oct 27 11:53:42 EST 1999


Protein A will definitely not do the trick. It binds only to a small
portion of the Fc domain, and won't block a significant portion of your
secondary antibody. What you need to do is make Fab fragments using
pepsin. Protocols are available readily in textbooks. 

Glen Tamura

On Wed, 27 Oct 1999, Goran Pavlakovic wrote:

> 
> Hi there,
> 
> does anybody have an idea how could I block/destroy Fc domain of an
> antibody (primary antibody) so that it would not be recognised by
> (otherwise) appropriate secondary antibody?
> 
> Would incubation with Protein A do the trick?
> 
> Thanx,
> 
> Goran
> 
> pavlakovic at gmx.net
> 
> 




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