How to block/destroy Fc domain of an antibody?

Goran Pavlakovic goran.pavlakovic at uni-essen.de
Wed Oct 27 15:13:49 EST 1999


First of all, thanx to all that answered to my question so far (hope there
will be more :-).
And now a second question:  What about biotinilation?

Goran

Glen Tamura schrieb:

> Protein A will definitely not do the trick. It binds only to a small
> portion of the Fc domain, and won't block a significant portion of your
> secondary antibody. What you need to do is make Fab fragments using
> pepsin. Protocols are available readily in textbooks.
>
> Glen Tamura
>
> On Wed, 27 Oct 1999, Goran Pavlakovic wrote:
>
> >
> > Hi there,
> >
> > does anybody have an idea how could I block/destroy Fc domain of an
> > antibody (primary antibody) so that it would not be recognised by
> > (otherwise) appropriate secondary antibody?
> >
> > Would incubation with Protein A do the trick?
> >
> > Thanx,
> >
> > Goran
> >
> > pavlakovic at gmx.net
> >
> >




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