> Is manganese a divalent cation as is calcuim and magnesium? if so, try a
> little EDTA (0.5mM to 5 mM) and see if that doesnt work. If I am confused,
> and it is not a divalent cation, the please ignore this post !
>> PS. when making up EDTA as a stock, you will have to pH it to get it to go
> into solution. EDTA is made up as a 0.5 M solution, but it will not go into
> solution without pHing to about 7.5 or 8 or so. It will not (to my
> knowledge) go into solution at higher concentrations.
Use Na-EDTA. Much easier.