EDTA in bacterial cultures
l.a.robertson at stm.tudelft.nl
Mon Sep 13 03:47:54 EST 1999
Russell Farris <tryggvi at email.msn.com> wrote in message
news:uxtjYS###GA.305 at cpmsnbbsa05...
> Is EDTA often used in culturing bacteria, and if so, what is it
> for. Thank you.
EDTA is often used as a chelator in defined mineral salts media to prevent
trace elements from forming insoluble precipitates (run a search on
"Vishniac & Santer" to get an example of such a trace element solution). A
small number of bacteria can be inhibited by EDTA, even at the
concentrations used in these media - iron citrate is sometimes another
option as a chelator, but not if your bugs like to eat citrate as it then
screws up your carbon balances.
More information about the Microbio