EDTA in bacterial cultures

Russell Farris tryggvi at email.msn.com
Mon Sep 13 12:09:56 EST 1999


Thanks, Lesley.  I'm interested in EDTA because Kazuyama et al. (1997) were
able to increase inclusion body-forming activity of Chlamydia
pneumoniae10,000 fold by use of EDTA or trypsin. I'm interested in EDTA
because it is a common
food additive, and I wonder what effect it has on the growth of C.
pneumoniae in the human body.

Russell Farris

Lesley Robertson <l.a.robertson at stm.tudelft.nl> wrote
> Russell Farris <tryggvi at email.msn.com> wrote ..
> >         Is EDTA often used in culturing bacteria, and if so, what is it
> used  for. Thank you.
> >
> EDTA is often used as a chelator in defined mineral salts media to prevent
> trace elements from forming insoluble precipitates (run a search on
> "Vishniac & Santer" to get an example of such a trace element solution). A
> small number of bacteria can be inhibited by EDTA, even at the
> concentrations used in these media - iron citrate is sometimes another
> option as a chelator, but not if your bugs like to eat citrate as it then
> screws up your carbon balances.
> Lesley Robertson
> Delft






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