Method for detection of viable spore

gerne at my-deja.com gerne at my-deja.com
Wed Sep 15 18:38:25 EST 1999


You can measure spore-functions directly after rehydration as they
respond then to normal membrane integrity measurements. My method of
choice would be flow cytometry (FCM). The Army has develloped a number
of methods to quickly germinate spores. Once the rehydration has occured
dead spores are recognised by impaired membran integrity. Unfortunatelly
even of all the spores that have undergone phase change and are intact
not all will elongate or polarize and replicate. Also there will be a
remaining fraction of spores that do not even undergo the phase change
in the first place. They can potentially also be quantified and tested
for extracellular enzyme functions using FCM and fluorescent probes.

In article <s7df6247.064 at dakghar.teri.res.in>,
  aloka at teri.res.in ("Alok Adholeya") wrote:
> This is a MIME message. If you are reading this text, you may want to
> consider changing to a mail reader or gateway that understands how to
> properly handle MIME multipart messages.
>
> --=_6B3D8EFF.2D4C225E
> Content-Type: text/plain; charset=US-ASCII
> Content-Transfer-Encoding: quoted-printable
> Content-Disposition: inline
>
> Dear Netters ,
>
> Is there a method to distinguish viable, non viable and viable but
dormant =
>  AM spores .
>
> Thanks in advance
>
> Dr. Alok Adholeya
> Fellow&Area convenor
> Centre for  Mycorrhizal Research
> TERI
> Darbari Seth Block
> Lodhi Road
> New Delhi - 110 003
> Tel: 91-11-4601550, 91-11-4622246 (Office)
>        91-11-5086772 (Residence)
> Fax: 91-11-4621770, 91-11-4632609
> Email: aloka at teri.res.in
>
> **************************************
> 1974-1975 to 1999-2000
> 25 years of innovation and change
> TERI's silver jubilee
> **************************************
>
> --=_6B3D8EFF.2D4C225E
> Content-Type: text/x-vcard
> Content-Transfer-Encoding: quoted-printable
> Content-Disposition: attachment; filename="Alok Adholeya.vcf"
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Adholeya, Alok
> ORG:;Microbial biotechnology
> EMAIL;WORK;PREF;NGW:aloka.TERIDL.TERI
> N:Adholeya;Alok
> TITLE:Fellow
> X-GWUSERID:aloka
> END:VCARD
>
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Adholeya, Alok
> ORG:;Microbial biotechnology
> EMAIL;WORK;PREF;NGW:aloka.TERIDL.TERI
> N:Adholeya;Alok
> TITLE:Fellow
> X-GWUSERID:aloka
> END:VCARD
>
> --=_6B3D8EFF.2D4C225E--
> ---
>


Sent via Deja.com http://www.deja.com/
Share what you know. Learn what you don't.



More information about the Microbio mailing list