Micococcus luteus cloning

dryden at snu.ac.kr dryden at snu.ac.kr
Tue Apr 11 07:36:39 EST 2000

I'm doing shotgun cloning of antibiotics-resistant determinant from Micococcus luteus, gram-
positive bacteria.
But I have failed so far.
I need helps to know whether M.luteus gene is well cloned or expressed in E.coli, whether there 
is good vector for gram-positive cell cloning.
I need any advices for cloning.

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