Here are the ingredients for Nutrient Broth proportions are for 1000ml Peptone 10gram Beef/Yeast Extract 3gram Sodium Chloride 5gram Distilled Water 1000ml Ph 7-7.2 If you want to make agar just add 30gram agar-agar to the above fluid. This makes Nutrient Agar. ashwin kelkar Sent via Deja.com http://www.deja.com/ Before you buy.