Bacillus sporulation

Walter Blank blank at marvin.tufts.edu
Mon Aug 7 09:59:01 EST 2000


Hi all,
I'm trying to find procedures for inducing sporulation in Bacillus (B.t.
or subtilis), separating out vegetative cells, and enumerating the
spores by cfu's (so I guess they'd have to be effectively germinated).
If anyone could pass along or point me in the direction of favorite
methods I'd appreciate it.
Thanks,
Walter Blank


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