DNA extraction from polyacrylamide gels

Larsen, Lise LarsenLi at missouri.edu
Wed Aug 16 11:21:43 EST 2000


How do you extract DNA from a polyacrylamide gel?

I run DGGE gels and need to excise the bands for PCR reamplification and 
sequencing. I've tried cut out  bands and freeze/thaw them ×3 in water but
didn't get 
any product after the following PCR. Please, let me know if you know of a
good 
method to recover the DNA.

Thank you
Lise Larsen



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