cheap probe to detect log growth

Peter C. Giardina, Ph.D. pgiardin at blue.weeg.uiowa.edu
Sat Dec 2 15:37:39 EST 2000


On Sat, 2 Dec 2000, Michael Witty wrote:

> Dear Peter, I use E. coli only, so I could just dip the probe in dilute
> ethanol before use.  Actually nothing more that washing up liquid might
> be OK!  What do you do at the Department of Microbiology?
> Mike
> 
> On Sat, 2 Dec 2000, Peter C. Giardina, Ph.D. wrote:
> 
> > On Sat, 2 Dec 2000, Michael Witty wrote:
> > 
> > > Dear All, I am convinced there must be a better way of finding the log
> > > phase of growth (for example of a conical flask of E. coli) than
> > > repeatedly taking 1ml samples and putting them in a spec.
> > > 
> > >      My ambition is to have a cheap detector with a probe consisting of a
> > > light source and photoresistor which can be dangled into my flask and
> > > record data continuously.
> > > 
> > >      But I am a biologist not an electrical engineer.  Does anyone share
> > > my ambition?  Or know how I can make this happen?  Mike.
> > > 
> > > 
> > 
> > Hmmm... Not sure how you'd sterilize the thing by a convenient method
> > without destroying it. And some folks have to shake their cultures pretty
> > hard.  It would have to be one tough little device.
> > 
> > Peter
> 


I'm a bacteriologist.  I work with pathogenic Neisseria species.

Regarding the probe device, you may try using side-arm culture flasks
which can be inserted directly into a spec.  It isn't continuous
monitoring, but better than opening your flask to take samples at time
points....reduced the risk of contamination.

Good luck!

Peter


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