Mueller-Hinton agar

Frederic Langlois at
Thu Dec 7 10:26:00 EST 2000

I finally found out (to late I guess since my exam was last week) that
Mueller-Hinton media doesn't contain PABA (para aminobenzoic acid) which
interferes with antibiotic diffusion in the medium.

I took me a while to figure it out because many many books only stated it
was used for antibiotic screenning because it's a standard... not really
helpful ::|

thanks for replying though ::)


> On Wed, 06 Dec 2000 19:01:20 -0800, Toai T NGUYEN <ttnguyen at>
> wrote:
> >I really do not know why Mueller-Hinton is preferred over other media for
> >screening of antibiotic resistance.  As far as I am concerned, if it
> >broke, don't fix it!
> >
> As someone else noted briefly, MH is used because it has become the de
> facto standard. At that point, no rational reason is needed, and no
> one may remember any original reason, if there is one. But for
> consistency, it is declared standard.
> bob

More information about the Microbio mailing list