pUC and pSC101

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Dec 11 10:51:02 EST 2000

In article <Pine.SGI.3.96.1001211130103.13655684C-
100000 at mole.bio.cam.ac.uk>, the eminent Michael Witty at University of
Cambridge, England wrote
>Dear All, I am trying to put a pUC based vector and a pSC101 based vector
>into one E. coli strain.  I think that pUC and pSC101 belong to different
>inc groups, but can someone reassure me?  Regards, Mike.

Correct. They should go in together without problems. Make sure you
don't have an F' with the same antibiotic selection as one of your
plasmids in your host strain, invariably Tet or Kan.  

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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