X-Gal

Gary G. Wilson ggw5 at columbia.edu
Thu Dec 14 11:40:13 EST 2000


Can someone give me some idea of how to do an X-gal stain on a bacterial
plate?  I've been given an old reference to Miller but don't have time to
wait to get it on interlibrary loan.

Do I add the X-gal to the agar?  How much?  How long does it take before I
see blue cells (typically)? Do I need to add anything else or just
X-gal etc.

A pointer to a contemporary reference  which detials this method would
suffice if nobody wants to give a verbose reply.

Thanks

Gary G Wilson (attempting to learn some microbiology)






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