help need of extinction coef of p nitrophenyl

philippe giammarinaro philippe.giammarinaro at adelaide.edu.au
Tue Dec 19 20:39:45 EST 2000


I m sorry (and i will surely loock stupid for that), nut i do not
understand. I lost my notion of biochemystry long time ago, and i do not
see how calculate the extinction coefficient.
Please help me.
philippe

Roberto Rosati wrote:
> 
> Hi Philippe,
> I think the best way for you to obtain this data - as well as a good
> standard curve - is to use p-nitrophenol as a reference standard, at the
> same conditions you're using in your assays.
> (Of course, remember to control again your pH after addition of
> p-nitrophenol, since it could change at high concentration, and it would
> alter the e.c.)
> Have good work!
> 
> -Roberto
> 
> ----- Original Message -----
> From: philippe giammarinaro <philippe.giammarinaro at adelaide.edu.au>
> To: <microbio at hgmp.mrc.ac.uk>
> Cc: <bionet.biology at adelaide.edu.au>
> Sent: Monday, December 18, 2000 11:27 PM
> Subject: help need of extinction coef of p nitrophenyl
> 
> >   hey,
> > I m using p-nitrophenyl
> > alpha-d-glucopyranoside to quantify the
> > activity of
> > alpha glucosidase i have in my samples, but i
> > miss an important data: What
> > is the millimolar extinction coefficient of
> > p-nitrophenyl at 410nm et at
> > 37o C and pH 6.6?
> > Thank in adavance for your respons.
> > Philippe Giammarinaro
> >
> 
> ---






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