Staph DNA Extraction
roeffner at csusm.edu
Wed Dec 20 13:23:38 EST 2000
I am looking into the ways to lyse/extract DNA from gram + cells. As far
as I can tell, the Promega Wizardprep kit is simple do to and the
extraction for G+ is the addition of lysozyme *(10mg/ml) @ 60 uL in a
300 uL extraction. Then incubate for 30-60 minutes @ 37C. If you are
having trouble lysing the staph, you can also add lysostaphin @ the same
concentration as the lysozyme (and in concert with it). This should lyse
almost any Staph cell.
That's what I could find out.
CSU San Marcos
Justin Ketchum wrote:
> Hi everyone! It's me again... I need to know how to
> extract DNA from Staph epidermidis. thanx. RSVP
> Justin Ketchum
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