Staph DNA Extraction

Richard Oeffner roeffner at csusm.edu
Wed Dec 20 13:23:38 EST 2000


I am looking into the ways to lyse/extract DNA from gram + cells. As far 
as I can tell, the Promega Wizardprep kit is simple do to and the 
extraction for G+ is the addition of lysozyme *(10mg/ml) @ 60 uL in a 
300 uL extraction. Then incubate for 30-60 minutes @ 37C. If you are 
having trouble lysing the staph, you can also add lysostaphin @ the same 
concentration as the lysozyme (and in concert with it). This should lyse 
almost any Staph cell.

That's what I could find out.

Richard Oeffner
CSU San Marcos

Justin Ketchum wrote:

> Hi everyone! It's me again... I need to know how to
> extract DNA from Staph epidermidis. thanx. RSVP
>                                    Justin Ketchum
> 
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