Allison M Arking amarking at
Sun Feb 13 22:38:05 EST 2000

I have found that poly-L-lysine works rather well as an adherence matrix.
Just a thought.

Jamie Arking, Ph.D.

Martin Weber < at> wrote in message
news:388C9C77.EEF0BB06 at
> Hi folks!
> Gotta grow embryonic neurons on coverslips for fluorescence microscopy
> experiments with fura-2 or fluo3.
> Problems:
> 1) Neurons don't grow very well on (poly-l-ornithine coated) GLASS
> coverslips.
> 2) Some (all???) PLASTIC coverslips show autofluorescence which can
> confuse the fluorescence measurements.
> Would be very glad about any suggestions, hints etc.
> Regards, Martin

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