NEURON CULTURES ON COVERSLIPS???

Allison M Arking amarking at home.com
Sun Feb 13 22:38:05 EST 2000


I have found that poly-L-lysine works rather well as an adherence matrix.
Just a thought.

Jamie Arking, Ph.D.


Martin Weber <martin.weber at urz.uni-heidelberg.de> wrote in message
news:388C9C77.EEF0BB06 at urz.uni-heidelberg.de...
> Hi folks!
>
> Gotta grow embryonic neurons on coverslips for fluorescence microscopy
> experiments with fura-2 or fluo3.
>
> Problems:
>
> 1) Neurons don't grow very well on (poly-l-ornithine coated) GLASS
> coverslips.
>
> 2) Some (all???) PLASTIC coverslips show autofluorescence which can
> confuse the fluorescence measurements.
>
> Would be very glad about any suggestions, hints etc.
>
> Regards, Martin






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