DNA is NOT a helix

Hiranya S. Roychowdhury hroychow at nmsu.edu
Wed Feb 16 19:05:28 EST 2000


I have GOT to do this experiment (!?)... 
Will you be kind enough to send me a protocol/idea?
If I could prove that DNA is not a helix, using just a plasmid and a
submarine gel apparatus... I have to see this thing through!

At 02:57 PM 2/16/00 EST, biegel at concentric.net wrote:
>I am new to user groups, and I hope therefore that this message reaches
>someone who is interested.  I seek a brave person with a DNA laboratory.
>
>I can prove that Form I DNA (i.e., "CCC" or covalently closed circular,
>duplex DNA) from native sources is generally NON-helical.
>
>Actually, this has been proven twice before, therefore I should say rather
>that I can prove DEFINITIVELY that native circular DNA is NOT, topologically
>speaking, a helix.
>
>But I can't do the work myself, because I have no lab, and I'm not a
>professional scientist.
>
>Anyone getting involved in this work stands to get famous, but also stands
>to be rather severely criticized.  People who challenge the "right-handed
>helix" tend to be ridiculed, and, if it's possible for them to be hurt
>(i.e., by cutting off of grant support), they may be hurt also.
>
>Therefore, I said above that I seek someone "brave", because you WILL be
>criticized for telling the truth.
>
>I also need someone with a laboratory.  To do the critical experiment you
>need access to good-quality (i.e., un-nicked) Form I circular DNA from a
>virus or plasmid, a pH meter, and apparatus for agarose gel electrophoresis.
>That's it!
>
>The experiment I will describe is so incredibly simple, you just won't
>believe that in 30 years, no one has seen fit to do it.  It takes 1 day.
>
>If you are interested, please contact me.
>
>Yours truly,
>
>K. Biegeleisen, M.D., Ph.D.
>
>
>
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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