isolation Bacillus thuringensis

panne at ubaclu.unibas.ch panne at ubaclu.unibas.ch
Thu Feb 24 09:31:14 EST 2000


We have tried to isolate BT from soil samples using the protocol from R.S.
Travers et al. (1987, Appl. Env. Microbiol, 53:1263-1266) without much
success. The method uses a heat selection and spore outgrowth in medium
containing 0.25 M sodium acetate. Apparently, BT does not sporulate under
these conditions and can therefore be enriched by subsequent heat
selection.

Did anybody use this method successfully? Does anybody have a better BT
isolation protocol or an idea from which sources we may have more luck?

Thanks,

Daniel




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