colony pcr question

Glen Tamura gtamura at u.washington.edu
Thu Jan 6 18:41:59 EST 2000


Colony PCR on gram positive organisms can be quite different than from
gram negatives. In particular, gram positive cocci have a very thick cell
wall and often need to be digested with lysozyme in an appropriate buffer
before transferring a small quantity of this to the pcr reaction. 

Glen Tamura

On Thu, 6 Jan 2000, noodle wrote:

> I'm looking for any protocols for pcr'ing genomic dna from a colony of
> G+ bacteria
> 
> For example, do I need a boiling step before I begin the pcr? (etc.)
> 
> -Kelli
> 
> 
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