PCR

Jan-Henner Wurmbach wurmbach at uke.uni-hamburg.de
Mon Jan 24 12:44:08 EST 2000


Beat schrieb im Beitrag <388ac948.830903 at news.ethz.ch>...
> Hi!
> I used to do that by extracting the bands and perform a dot blot.
> Subcloning and sequencing would be the other solution...
> 
> Beat
> 
> 
> 
> On Sun, 23 Jan 2000 15:47:26 +0800, "Chong Wai Yin( Zhang Weixian)"
> <wychong at singnet.com.sg> wrote:
> 
> >Hi, is there anyway to know if the extra bands after PCR is due to
> >unspecific binding or due to fragmented DNA-of-interest. (eg. gene of
> >interest is fragmented and after PCR, the fragment is amplified.).
Thanks.
> >
You can Cycle sequence your PCR-Fragment with the PCR primers. I do it with
ABI-Big-Dye Kit and it Works with Gel purified (Quiagen) Fragment. (At
least one day less than subcloning).

Greetings 
  Jan-Henner Wurmbach





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