NEURON CULTURES ON COVERSLIPS???

Martin Weber martin.weber at urz.uni-heidelberg.de
Mon Jan 24 13:39:51 EST 2000


Hi folks!

Gotta grow embryonic neurons on coverslips for fluorescence microscopy
experiments with fura-2 or fluo3. 

Problems:

1) Neurons don't grow very well on (poly-l-ornithine coated) GLASS
coverslips.

2) Some (all???) PLASTIC coverslips show autofluorescence which can
confuse the fluorescence measurements.

Would be very glad about any suggestions, hints etc.

Regards, Martin




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