Hi, I have immunised a rabbit and I was wonderign what kind of titers you can expect. I tested the serum and I had to dilute the serum 1:100.000 at least to get a reasonable ELISA-curve (even at 1:1000000 there is till a good signal). When I dilute it less the color in the ELISA is brownish instead of bright yellow (so way too dark). Is this normal?? Suzanne