DNA contanmination in RNA prep.

l ddp95120NOddSPAM at yahoo.com.invalid
Fri Jul 7 12:02:52 EST 2000


I'm trying to look for a efficient method to remove the DNA
contanmination in my total RNA samples. I'm working with a
Gram-positive bacteria E. hirae and I'm trying to use the RNA for
quantitative RT-PCR. I've used both the Gaunidium thiocyanate
method and the Qiagen's RNeasy kit to extract my RNA. I then
treated the RNA with DNase I. However, my no RT control kept
giving me positive signal in my PCR. I'll appreciate it very much
if someone can give me an advice. Thank you.




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