Western blotting question

Jim Hu jimhu at tamu.edu
Wed Jun 21 02:23:27 EST 2000


DAB works well, but  I think it's a carcinogen, so handle with care.  The
Pierce site also lists a precipitating TMB substrate that they say is safer
and doesn't use peroxide - search for TMB on the web site.  I haven't used
it, but their stuff is generally pretty good.

Suzanne Hermeling wrote:

> Hi
>
> I have a small question about western blotting. I have a substrate that
> reacts with GARPO to give a soluble blue product (i.e. TMB-dichloride).
> I have always used this in an ELISA. However I want to run Western Blots
> as well.
>
> Does anyone know whether I can use the same substrate or do I have to
> get a product that results in a non-soluble product?
> I have searched on the web-page of Pierce and they say that the soluble
> substrate is for ELISA and they don't say anything about Western
> Blotting with this substrate.
>
> Can anyone help me??
>
> Suzanne






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