growth on continuous culture vessel walls

David B. Hedrick davidbhedrick at
Thu Mar 23 23:14:38 EST 2000

If it wants to attach, let it.  Increase the surface area (beads,
parallel plates).  Grow to a steady-state biofilm.  Get yields by
measuring biomass of the effluent.  Much more difficult, but truer to
the organism.  

bo wrote:
> Hi,
> I'm currently growing Chl. tepidum (an anaerobic green sulfur bacterium) in
> continuous culture at a dilution rates of 0.15 h-1 and using the Wahlund's
> medium (Wahlund et al., 1991; Arch. Microbiol. 156:81-90). The main problem
> is that it heavily attachs on the vessel walls, which makes impossible
> further calculations on light intensity available, cell yields, etc. I know
> that the problem is not new but I don't know for the moment how to avoid it.
> If anybody knows how to solve it (maybe it is enough with a slight
> modification of the growth medium?), please, don't hesitate to contact me.
> Carlos
> _______________________________________
> Carles M. Borrego
> Lab. Microbiology, Institute of Aquatic Ecology
> University of Girona
> Campus de Montilivi, E-17071, Girona (Spain)
> Phone:  +34 972 418175
> FAX:     +34 972 418150
> _______________________________________


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of chemistry and biology. 

	davidbhedrick at

	David B. Hedrick
	P.O. Box 16082
	Knoxville, TN 37996

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