growth on continuous culture vessel walls

David B. Hedrick davidbhedrick at icx.net
Thu Mar 23 23:14:38 EST 2000


If it wants to attach, let it.  Increase the surface area (beads,
parallel plates).  Grow to a steady-state biofilm.  Get yields by
measuring biomass of the effluent.  Much more difficult, but truer to
the organism.  


bo wrote:
> 
> Hi,
> 
> I'm currently growing Chl. tepidum (an anaerobic green sulfur bacterium) in
> continuous culture at a dilution rates of 0.15 h-1 and using the Wahlund's
> medium (Wahlund et al., 1991; Arch. Microbiol. 156:81-90). The main problem
> is that it heavily attachs on the vessel walls, which makes impossible
> further calculations on light intensity available, cell yields, etc. I know
> that the problem is not new but I don't know for the moment how to avoid it.
> If anybody knows how to solve it (maybe it is enough with a slight
> modification of the growth medium?), please, don't hesitate to contact me.
> 
> Carlos
> _______________________________________
> Carles M. Borrego
> Lab. Microbiology, Institute of Aquatic Ecology
> University of Girona
> Campus de Montilivi, E-17071, Girona (Spain)
> Phone:  +34 972 418175
> FAX:     +34 972 418150
> _______________________________________

-- 
		~DBH

Technical writing, literature search, and data analysis at the interface
of chemistry and biology. 

	davidbhedrick at icx.net

	David B. Hedrick
	P.O. Box 16082
	Knoxville, TN 37996




More information about the Microbio mailing list