I need help

lamb L.A.M.Buisman at cable.A2000.nl
Sat Mar 25 14:06:40 EST 2000

David Lawton wrote:

> It's 1 o'clock in the morning here and I'm doing this because I can't be
> bothered to rewrite an SOP for our autoclaves,

When you're finished, can I have it? :-))

> so can we leave works like oxymoron out of this. ;o)
> With your NaCl example, you're selecting not enriching. If you had a broth
> culture then you've selectively enriched.
> You mean people look for bacteria that have no human association? ;o))
> We could go around in circles here, it's probably not so much how the media
> is used as the choice of media, if that makes sense.
> The obvious question is, do people use blood agar selectively?

I doubt that.

> What is R2A agar anyway, probably called something different over here.

I don't know the composition. The only reason why we use it is because it is
mentioned in the official guidelines for bacterial control of haemodialysis
fluids. We get it from Oxoid, it is in the manual.
With R2A and TSA total counts are performed, to detect possible pathogens
(coliforms, Pseudomonas) blood agar could be used (in that sense it could be
called selective) but we prefer CLED agar.

> David Lawton
> And the next thing I'll demonstrate is that black is white, white is black
> and get killed on the next pedestrian crossing.

Never step on the black stripes!!



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