Decreased plasmid production

David Dai kdai at hgmp.mrc.ac.uk
Thu May 4 04:44:38 EST 2000


Dear all,

Recently, I am doing truncation assay on my interested promoter fragment. 
I am using pGL3 reporter.  When the truncation came to close to the
transcription start site, the generated plasmid production is strangely
decreasing from every continous culture. Of course, the bacteria growth
looks as thick as usual. Whoever can give any sugestion or idea of this
event will be appreciated.

WBR

David





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