Different counts in MacConkey Agar
kees.lyn at xtra.co.nz
Mon May 29 07:19:18 EST 2000
> "Lynn" <kees.lyn at xtra.co.nz> wrote in message
> news:3930ECAC.728D at xtra.co.nz...
> > david.lawton1 wrote:
> > >
> > > "Yjgent" <yjgent at aol.com> wrote in message
> > > news:20000526213618.02915.00000277 at ng-fz1.aol.com...
> > > > >In this lab we're working with E.Coli (ATCC 25922 and wild type) and
> > > > >routinely use MacConkey Agar inclusion plating as a counting method
> > > > >these microorganisms.
> > > >
> > > > I was taught a long time ago NOT to measure a colony count from a Mac
> > > plate.
> > > > Only a non selective agar like TSA or a Blood Agar plate can be used
> > > count
> > > > colonies.
> > > > John Gentile M(ASCP) President - Rhode Island Apple
> > > Group
> > > > yjgent at aol.com
> > > > Microbiologists do it with culture.
> > > Depends on the formulation and what you are trying to count.
> > > For years we used a MacConkey that surpressed gram - positives combined
> > > a blood agar for urine.
> > > You can use Mac for water counts, though not recommended
> > >
> > > Dave
> > Why is it not recommended for water counts, what would be more suitable?
> > Lynn
> Membrane lauryl sulphate broth, presuming your looking for E. coli or
Thanks Dave, our lab may be doing water testing soon and Im just
starting to sort out what tests to use.
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