mutational bacterial strains

Helen Niederwald helennie at
Tue Nov 14 16:38:06 EST 2000

you may want to check medline for "mutS" mutants of e.coli. if i
remember correctly, growing your plasmids in that type of strain
increases the probability for random mutations.


In article <Pine.GSO.4.10.10011141202430.770-
100000 at>,
  "Gary G. Wilson" <ggw5 at> wrote:
> I'm new to the field and am looking for references to definitive
> on methods for using mutational strains of bacteria to create random
> mutations in a plasmid.  Can anyone point me in the right direction.
> Gary G Wilson

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