enzymes against bacteriocins
internul1 at hotmail.com
Tue Nov 21 11:30:36 EST 2000
for a work on bacteriocin production from lactic acid bacteria, I use the
deferred antagonism assay with good results. Many isolated strains (116 out
of 1000) inhibit the target bacteria (Listeria) on solid media (BHI, TSA-YE,
However, I cannot succeed to stop the inhibition by adding several
proteolytic enzymes commonly used for this purpose.
The enzymes (protease, trypsine, proteinase K) are solubilized in PBS 0.01M
at 10mg/mL, and a few ug are deposed on spots of lactic cultures on solid
media, and an overlay is added containing Listeria. But the inhibition zones
are still present. Obviously, maybe there are no bacteriocin-producing
strains, but what can cause the inbition (clues: no peroxyde because
anaerobiosis; no bacteriophages detected; no acidification because of the
no-glucose TSA-YE) ? Is this enzyme methodology inadequate ?
Thank you for help !
Center for aquatic innovation
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