yjgent at home.com
Wed Nov 22 22:35:48 EST 2000
Pantoea agglomerans (formally known as Enterobacter agglomerans) is (IMHO) a
difficult organism to pin down. I always thought that the name "agglomerans"
meant that is was an agglomeration of a bunch of related species that
couldn't be defined. Many of the reaction charts list +/- reactions for many
substrates. I've found that the API 20E strips did a very good job at
distinguishing all the Enterobacters, and was often frustrated with the
Vitek GNI card. I think the GNI+ card is a little better, but would still
trust the API. I think the MicroScan does OK with the Enterobacters, but
still not as good as API. (I'm not trying to sell API - honest!)
I also remember a long forgotten test for Enterobacters - the KCN test
(that's potassium cyanide) - of course that was back in the days of mouth
pipetting and I'm still here!
John Gentile Rhode Island Apple Group
yjgent at home.com Past President, Publicity Chairman
"I never make mistakes, I only have unexpected learning opportunities"
> From: bqglcd at yahoo.com (Bernie Quinn)
> Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
> Newsgroups: bionet.microbiology
> Date: 22 Nov 2000 02:10:41 -0000
> Subject: Pantoea
> Is anybody familiar with the genus Pantoea?
> How many species are there?
> My question is - does Pantoea genus produce gas in
> lauryl tryptose broth? Is there any decisive
> information anywhere that would confirm that the genus
> is coliform, or is there only information on Pantoea
> Please help, we have an organism that has been
> identified as two different genera, one of which is
> pantoea. The problem is we got gas produced in lauryl
> tryptose broth, gas in brilliant green and green
> metallic sheen on m-endo, but the identifying lab
> didn't get gas in lauryl tryptose from the slant which
> we isolated and only identified the bug as pantoea (no
> species) which they claim is not a coliform.
> Any ideas, anyone??
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