E. coli strain B

Helen Niederwald helennie at my-deja.com
Tue Nov 28 08:35:28 EST 2000


In article <3A22DB2F.489D0F0A at princeton.edu>,
  gerchman at Princeton.EDU (Yoram Gerchman) wrote:
> Greetings netters
> I have a few questions about E. coli strain B (WU36):
> I want to grow it on minimal medium A, do I need to add Thiamine?

Yes, most likely.

> This is an auxtrof mutant (tyrA14), how much tyrosine shuld be added?

Well, try it. Set up a couple of liquid cultures and add different
amounts of TYR. I would guess you will need TYR in the low micromolar
range (50 -100 uM).
Regards,
Helen.




> Thanks Yoram
> ------------------------------------------------------------
> "Support bacteria - they're the only culture some people have"
>
>                                      (anonymous)
>
> ---
>


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