Mueller-Hinton agar

Richard Oeffner roeffner at
Wed Nov 29 17:14:44 EST 2000

According to Difco Manual, from Nat'l Comm. for Clinical Lab Stds. MH 
agar is used in antibiotic assays because it shows good batch to batch 
reproducibility; low in sulfonamide, trimethoprim and tetracycline 
inhibitors; good growth of most non-fastidious organisms; and lots of 
data available from previous testing.

Basically, it seems that it has become the standard because of all of 
the previous work done in antibiotic assays done with it, and you can 
reproduce such resuslts repeatedly...always a plus if you're 
standardizing things.

Richard Oeffner
Cal State San Marcos

Magic Chicken wrote:

> Hi, I'm a biomedical student @ UQTR in Trois-Rivieres, PQ, Canada and I'm
> trying to find out why do we use Mueller-Honton media to determine the
> sensibility to an antibiotic. From what I've found so far, it's only a
> nutritive media, without any inhibitors... Is there any other reason to
> choose a M-H agar over a TSA agar (for example)?
> TIA,
> Frederic Langlois

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