Bacillus stearothermophilus

David Studholme D.J.Studholme at soton.ac.uk
Thu Nov 30 05:38:35 EST 2000


In response to Rene's question about transforming B. stearothermophilus - I had big problems
transforming this bug a few years ago (and in fact appealed for help in this newsgroup and recieved some very helpful replies).

Anyway, I found the method that worked best was Issay Narumi's electroporation method (published in
Biotechnology LettersI think).



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David J. Studholme, PhD.

Division of Biochemistry & Molecular Biology
School of Biological Sciences
University of Southampton
Bassett Crescent East
SOUTHAMPTON
SO16 7PX
UK

djs at soton.ac.uk
http://promscan.uklinux.net
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