RGD and SGxG bacterial adhesion assay

Richard P. Grant rpg14 at yahoo.co.uk.invalid
Mon Oct 2 08:01:02 EST 2000


In article <rh-0210001209320001 at a6-curtis.ibls.gla.ac.uk>, 
rh at mblab.gla.ac.uk (Robert Hartley) wrote:

>  To ovecome the
> problem of other bacterial adhesion sites interacting with the eukaryotic
> monolayer, one could use a small rgd containing peptide and do a
> compettitive inhibition assay.

You have to be careful with this - as some of the 'RGD' peptides don't 
have activity.  I think it's Gibco who sell a 'cyclic' RGD peptide which 
we found worked quite well.

If you have them, mAb frags might be a good competitor, too - and more 
specific for the effect he's looking at.

-- 
Richard P. Grant MAD Phil      http://www2.mrc-lmb.cam.ac.uk/personal/rpg/ 

Please reply to rpg 'at' mrc-lmb.cam.ac.uk'






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