freeze drying

[ianj]

Off the top of my head:

Grow the organism in skim milk + 1% yeast extract + 1% glucose.

After incubation to early stationary phase, subculture 10% into fresh medium and freeze dry.

regards

Ian Jenson
    "LynnB" wrote in message <39F94AE1.EA0039DF at ctech.ac.za>...
    I am about to commence freeze drying cultures of Bifidobacterium, and as I have never attempted to do this before, I am unsure as to what to do.  Any advice, eg the addition of skim milk powder etc, will be gratefully received. 
    Thanks, 

    (Dr)  Lynn Brown 

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