Yeast clone recovery from glycerol stock

Jackson, John C. JohnC.Jackson at anheuser-busch.com
Wed Sep 13 07:01:38 EST 2000


A good part of your recovery probably has to do with the amount of glycerol in
the frozen sample.  A 5% final glycerol concentration seems to give the best
results, but there may be some strain dependency involved.  Additionally, a
rapid thaw in a 35 deg water bath, seems to be important for maximum recovery of
viable cells.
Hope this helps,

--John


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