Yeast clone recovery from glycerol stock

[Anna]

Thanks for your advice!!! No wonder why they don't grow, the samples
were made with 20% glycerol concentration. Any remedy i can make?
Thanks again!

anna

"Jackson, John C." wrote:
> 
> A good part of your recovery probably has to do with the amount of glycerol in
> the frozen sample.  A 5% final glycerol concentration seems to give the best
> results, but there may be some strain dependency involved.  Additionally, a
> rapid thaw in a 35 deg water bath, seems to be important for maximum recovery of
> viable cells.
> Hope this helps,
> 
> --John
> 
> ---