What is primer extension and chromosomal walk technique

Justin Doward c9602805 at studentmail.newcastle.edu.au
Tue Apr 3 02:57:58 EST 2001


I thought that the whole idea of Taq polymerase was its thermostability?
Does the reverse transcriptase enzyme have greater stability at high temp?
Anyway apart from that you are right I was way off, I remembered sitting in
a lecture once and the mechanism by which the enzymes moved along dna
described something similar to walking and the primer extension I was just
thinking along the lines of primers in pcr etc... sorry about that Qin Lin.



Sergio <sergioal at bbm1.ucm.es> wrote in message
news:3AC8728A.2353B47F at bbm1.ucm.es...
>
>
> Justin Doward wrote:
>
> > Try looking up the polymerase chain reaction (pcr) for primer extension
> > information, and I think chromosomal walk technique is referring to the
way
> > in which enzymes (such as dna polymerase etc) move along dna so look up
DNA
> > replication.
> >
>
> !!??? Where did you find that information???
>
> Primer extension is a technique to find the transcription iniation
nucleotide.
> Its based on the amplification of the messenger RNA using the
retrotranscriptase
> (not a PCR enzyme, since none of them are termostable), and comparing the
size
> of the amplified cDNA with a sequence of the gene (both performed using
the same
> primer).
>
> Chromosome Walking is a technique to clone adjacent fragments of DNA from
a
> chromosome. There are different methods, based on labelled probes or
insertion
> of marker genes.
>
> Try any molecular techniques book...
>
> Sergio
>





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