bacterial spores to vegetative cells

[Martin Smith]

MAybe 85 degrees isint enough. I suggest a lower temperature for a longer
interval of time (i.e. 50-60 gedrees for 30 mins). If this doesnt work, he
spores are probably genetically defective or absent (I see no other
solution).

"Aldert Zomer" <zomeral at biol.rug.nl> wrote in message
news:3AB630A4.7419C3B8 at biol.rug.nl...
> Hi all,
>
> We're having some minor problems with the growth of vegetative cells
> from spores. We treat the spores with a heat shock, 85 oC for 5 minutes
> to induce growth but sometimes the spores just don't grow. (The spores
> in question are from clostridium beyerinckii and clostridium
> tyrobutyricum, found in cheese)
>
> Are there any other methods to induce the spores?
>
> --
> Aldert Zomer