Dcm- Rec A- strain ?

Eric DUFOUR Eric.Dufour at cmm.ki.se
Tue Apr 10 04:56:56 EST 2001


Dear all,

    It's my fifth catalog and it seems impossible for me to find an
approriate strain (dcm- recA- hsdR at least) (dcm- dam- hsdR  mcr-
recBC- sbcC- if possible). I need to use the Acc65I enzyme, an
isoschisomere of KpnI and this enzyme is Dcm semsitive. And my plasmid
starts to be large (app 10Kb). Does anyone know about such a strain ?

Thanks in advance,
Eric




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