Try contacting Life Technologies, I'm sure they make DMEM and RPMI without
"EddieWK" <ewkip at hkucc.hku.hk> wrote in message
news:9kc1cp$18502 at hkunae.hku.hk...
>> I have a question about medium used in tissue cultruing. I'm currently
> doing functional assays using RPMI 1640 medium, in which ligand binding on
> cells may occur. I would like to know if I want some "divalent cation"
> present in the medium to test the nature of binding, anyone can tell me
> chemical compound(s) I should add into the medium to provide the "divalent
> cation", like Ca++? or other kind of medium can be used instead of RPMI.
> Thank you in advance.