How to provide divalent cation in medium?

Patrick Druggan Patrick.Druggan at
Sat Aug 4 05:28:49 EST 2001

Try contacting Life Technologies, I'm sure they make DMEM and RPMI without
divalents. Pd.
"EddieWK" <ewkip at> wrote in message
news:9kc1cp$18502 at
> Dear,
> I have a question about medium used in tissue cultruing.  I'm currently
> doing functional assays using RPMI 1640 medium, in which ligand binding on
> cells may occur.  I would like to know if I want some "divalent cation"
> present in the medium to test the nature of binding, anyone can tell me
> chemical compound(s) I should add into the medium to provide the "divalent
> cation", like Ca++?  or other kind of medium can be used instead of RPMI.
> Thank you in advance.
> Regards
> Eddie

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